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| 【主 题】 ： |
Human Umbilical Vein Endothelial Cells (HUVEC) |
| 【联系人名】 ： |
Wendy |
| 【电子邮件】 ： |
contact@creative-bioarray.com |
| 【联系电话】 ： |
1-631-626-9181 |
| 【发布日期】 ： |
2015-1-26 |
| 【详细说明】 ： |
The vascular endothelial cells contribute to the
maintenance of vascular homeostasis. They synthesize
and secrete activators as well as inhibitors of both
the coagulation system and the fibrinolysis system
in addition to mediators that influence the adhesion
and aggregation of blood platelets. Endothelial
cells also release molecules that control cell
proliferation and modulate vessel wall tone. Many of
the endothelial processes can be studied in vitro
using cultured cells, and human umbilical vein
endothelial cells (HUVEC) are the most commonly used
cell type for such studies. Except for the common
endothelial cell features such as "cobblestone"
morphology, positive staining for Factors VIII and
the ability to take up acetylated low-density
lipoprotein , HUVEC stein positively with CD-31.
Cells pretreated with IL-1 or TNFalpha also
selectively express E-selectin and VCAM. HUVEC from
Bioarray Research Laboratories are isolated from
human umbilical veins. HUVEC are cryopreserved at
passage one and delivered frozen. Each vial contains
>5 x 10^5 cells in 1 ml volume. HUVEC are
characterized by immunofluorescent method with
antibodies to vWF/Factor VIII and CD31 and by uptake
of DiI-Ac-LDL. HUVEC are negative for HIV-1, HBV,
HCV, mycoplasma, bacteria, yeast and fungi. HUVEC
are guaranteed to further expand for 15 population
doublings at the conditions provided by Bioarray
Research Laboratories.
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English
