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| 【主 题】 ： |
Recombinant Human Cystatin F, Fc chimera |
| 【联系人名】 ： |
Candy Ton |
| 【电子邮件】 ： |
yuanyuanton@gmail.com |
| 【联系电话】 ： |
1-631-5599269 |
| 【发布日期】 ： |
2015-1-26 |
| 【详细说明】 ： |
Product Overview
Recombinant Human cystatin F encoding the human Cystatin F precursor (NP_003641.2) (Met1-His145) was produced in HEK293. It was expressed with the fused Fc region of human IgG1 at the C-terminus.
Description
The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.
Molecular Mass
The recombinant human Cystatin F/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 364 amino acids and predicts a molecular mass of 41.3KDa. As a result of glycosylation, the rh Cystatin F/Fc monomer migrates as an approximately 50KDa band in SDS-PAGE under reducing conditions.
Source
Human Cells (HEK293).
Predicted N Terminal
Gly 20.
Endotoxin
<1.0EU per 1μg cytokine as determined by the LAL method. |
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